Part:BBa_K3773521:Design

Circuit to report PdnaKJ expression in vivo

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 367
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We found that of various heat shock response genes, dnaK and dnaJ are two of the most frequently differentially expressed in E. coli upon transformation with a heterologous circuit. As a result, we use the dnaKJ operon promoter as one representative of promoters related to the heat shock response.

The sequence inputted as a scar after the RBS is a spacer. For our spacer, we use RiboJ, an insulator which has been shown to allow for expression with Bba_B0034 and sfGFP¹.

Source

See basic parts